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文献和实验were eluted with two 3-ml portions of elution buffer (Pierce kit); diluted to 13 ml with TBS; dialyzed at 4ºC against two changes of TBS and three changes of PBS (0.15 M NaCl,5 mM NaH2PO4,pH adjusted to 7.0 with NaOH); and stored at –20ºC.Protein concentration
v468 Chapter 9 自身启动子报告基因验证转录靶点
, S., Hlubek, F.,Kirchner, T. (1999) beta-catenin regulates the expression of the matrix metalloproteinase-7 in human colorectal cancer. Amer J Pathol 155 , 1033–1038. 7. Crawford, H.C., Fingleton, B.M., Rudolph-Owen, L.A., Goss, K.J., Rubinfeld
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
mitochondrial transition pore formation by binding cyclophilin D. The Image-iT LIVE Mitochondrial Transition Pore Assay Kit has been tested with HeLa cells and bovine pulmonary artery endothelial cells (BPAEC). Each Image-iT LIVE Mitochondrial Transition Pore
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