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文献和实验Assessment of Circadian and Light‐Entrainable Parameters in Mice Using Wheel‐Running Activity
, physiological variables are regulated by an internal clock. This endogenous circadian (?24?hr) clock enables organisms to anticipate daily environmental changes and modify behavioral and physiological functions appropriately. Processes regulated by the circadian
α response, we used two human-tumor-derived cell lines: the melanoma line ME-15 (10 ) and the hepatoma line HuH7 (11 ). We have chosen these cell lines as models, because we have a good understanding of the IFNα responses at the mRNA and the protein levels
【精华】vol 687 Chapter 5 PCR引物与PCR引物设计
cycles. We have developed an interactive web site andalgorithm (iCODEHOP) for designing CODEHOP PCR primers from multiply aligned protein sequences,which is freely available online. Here, we describe the workflow of a typical CODEHOP PCR assay designand
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