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- 详细信息
- 文献和实验
- 技术资料
- 宿主:
Mouse
- 克隆性:
多克隆
- 保存条件:
2-8℃
- 免疫原:
Donkey
- 规格:
C4064&D2193
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文献和实验Making Boiled Donkey (or whatever) Serum (BDS) for Blocking
aliquot, but maybe if it's a fresh aliquot use it at 1:400) for 30 minutes at 37oC. 11) Rinse 3 times for 5 minutes each rinse in PHEM-T. 12) Put on secondary (I use Donkey anti-mouse-Rhodamine Red X) at 1:100 in block for 30 minutes at 37oC. 13
Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
Brdu免疫组织化学染色分析 BrdU incorporation assay
of Rhodamine(Tritc) conjugated Donkey anti mouse IgG with 1% NDS,1%BSA in PBS, incubate at RT for 1 hr. Wash in PBS 3x Mount with 50% glycerol in PBS. Culture Cell Enzyme Immunostaining for BrdU: Wash plates with in PBS
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