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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Goat anti-Mouse IgG Fc Secondary Antibody, AP
- 靶点:
Mouse IgG Fc
- 浓度:
0.6 mg/ml
- 应用范围:
Western Blot
- 宿主:
Goat
- 适应物种:
Mouse
- 克隆性:
Polyclonal
- 保存条件:
4° C
- 形态:
Lyophilized
- 亚型:
IgG
- 规格:
1 mL
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文献和实验secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary antibody (Typically SBA goat anti rabbit-AP 1:100, rabbit anti goat-AP 1:300) 22- wash twice 23- [optional] add a tertiary layer (rabbit anti-goat-AP on a goat anti-rabbit-AP) 24- wash thrice 25- develop AP (see protocol
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