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- 2025年12月18日
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文献和实验Searching for Non‐B DNA‐Forming Motifs Using nBMST (Non‐B DNA Motif Search Tool)
basic protocols on using the non?B DNA Motif Search Tool (nBMST) to search for sequence motifs predicted to form alternative DNA conformations that differ from the canonical right?handed Watson?Crick double?helix, collectively known as non?B DNA
Isolation of Untouched Human B Cells by Depletion of Non-B Cells
实验原理 Add a mixture of biotinylated monoclonal antibodies (Antibody Mix) against the non-B cells to the starting sample. Add Depletion MyOne SA Dynabeads® to bind the non-B cells during a short incubation. Separate the bead
x dig/dNTP mix*: 1 mM dATP 1 mM dCTP 1 mM dGTP 0.65 mM dTTP 0.35 mM alkali-labile digoxigenin (dig)-UTP (B-Mann cat# 1573152 or 1573179) *supplied in the dig DNA labelling kit; also can order all tubes separately, i.e. without enzyme
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