- 询价
- Roche
- 进口
- 11088793001
- 2025年12月18日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询说明书
- 库存：
10
- 供应商：
上海远慕生物
- 规格：
2.5 g
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文献和实验sepharose 4B (Sigma) C-9142 5g. This amount swells to 20 ml volume . Swell resin in 10 mM HCl (86 ml of conc. HCl into 100 ml H2 O) for 15 min at room temperature. Have a scintered glass filter ready to wash resin. Pour the swelled resin into the filter
The Isolation and Purification of Rodent Pancreatic Islets of Langerhans
of Langerhans from the rest of the pancreas. The pancreas is excised from the animal, trimmed of non-pancreatic tissues before being inflated and chopped into small pieces. The connective tissue is then broken down with a collagenase enzyme solution
activity against the high concentrations of non-collagen proteins and other macromolecules found in the extracellular matrix. The collagenase most commonly used for tissue dissociation is a crude preparation containing clostridiopeptidase A in addition
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