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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
10x Uracil Cleavage System
- 供应商:
默瑞生物
- 规格:
750 Reactions
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. Isacson. 1992. Authentic and artifactual detection of the E. coli laxZ gene product in the rat brain by histochemical methods. Brain Research 16 :311. Snyder, E.Y., D.L. Deitcher, C. Walsh, S. Arnold-Aldea, E.A. Hartwieg, and C.L. Cepko. 1992
AFLP: not only for fingerprinting, but for positional cloning
acid from mini(micro)prep) 5 U EcoRI 5 U MseI 8.0 µL 5x-Pharmacia "One-Phor-All+" buffer [10x = 100 mM Tris-acetate, pH 7.5, 100 mM Mg-acetate, 500 mM K-acetate) w/ 250 ng/µg BSA (=5x OPA+- BSA) Q.S. to 40 µL w/ dH20 - incubate @ 37° C for ~ 3 hrs
) 10X assay buffer one-tenth volume DNA x μl restriction enzyme* y μl (1-10 units per ug DNA) Total volume z μl If desired, more than one enzyme can be included in the digest
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