The Human BRAF Gene qBiomarker Somatic Mutation PCR Array allows the detection of 20 unique mutations in the human BRAF gene. BRAF, a proto-oncogene and a serine/threonine protein kinase, regulates the MAP kinase/ERK signaling pathway and its role in affecting cell division, differentiation, and secretion. Previous scientific reports have shown a strong association between mutations in the BRAF gene and non-Hodgkin lymphoma, hairy cell leukemia, and malignant melanoma as well as cancers of the colon, lung, and thyroid. The BRAF mutations found in cancer samples are predominately missense point mutations that either increase or impair BRAF kinase activity, such as p.V600 and p.G469A mutations respectively. The DNA sequence mutation assays in the array detect the most frequent mutations in BRAF compiled from over 11,000 reported cancer sample sequences. Each mutation assay is bench-tested for the sensitive detection of a low percentage of mutation-containing DNA in a background of wild-type genomic DNA. The 96-well format of this array profiles the mutation status of 4 samples, while the 384-well array format profiles 16 samples, and each set of plates includes qPCR mastermix. Using real-time PCR, research studies can easily and reliably detect a panel of mutations in the BRAF gene with this array.