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Insulin Receptor-Associated Proteins: Insulin and Receptors: INS, INSL3, IRS1, IRS2, IRS4, SORBS1.
Insulin-like Growth Factors and Receptors: IGF1R, IGF2, IGFBP1.
SH3/SH2 Adaptor Protein: CBL, GAB1, GRB2, IRS4, NCK1.
Other Genes Associated with Insulin Receptor Proteins: CAP1, DOK1, DOK2, DOK3, FRS2, FRS3, INSR, PDPK1, PPP1CA, PTPN1, PTPRF, SHC1.
PI-3 Kinase Pathway: Components: AKT1, AKT2, AKT3, EIF2B1, EIF4EBP1, MTOR, GSK3A, GSK3B, PDPK1, PIK3CA, PIK3R1, PIK3R2, PRKCG, PRKCI, PRKCZ.
Target Genes: BCL2L1, DUSP14, G6PC, HK2, IGFBP1, PCK2, SERPINE1, SLC2A4, SREBF1, UCP1, VEGFA MAPK Pathway: Components: ARAF, BRAF, EIF2B1, GRB2, HRAS, MAP2K1, MAPK1, RAF1, RPS6KA1, RRAS, RRAS2, SHC1, SOS1.
Target genes: ANG, BCL2L1, ERCC1, FOS, LDLR, NOS2, UCP1.
Primary Target Genes for Insulin Signaling: CEBPA, CEBPB, FOS, JUN, LEP, PRL.
Secondary Effector Target Genes for Insulin Signaling: NPY, PCK2, SLC2A1, SLC2A4, TG.
Target genes for PPARg: ACOX1, ADRB3, AEBP1, CAP1, CEBPA, CEBPB, PCK2, PPARG, RETN.
Target genes for SREBP1: ACACA, FBP1, G6PC, GCK, PCK2, PKM2.
Carbohyde Metabolism: Glucose Metabolism: FBP1, G6PC, GCK, HK2, INS, IRS2, PCK2, PKM2, SLC2A1, SLC2A4, SORBS1.
Glycogen Metabolism: G6PC, GSK3B, PPP1CA.
Other Genes Related to Carbohyde Metabolism: ADRB3, GPD1, INSR.
Lipid Metabolism: Cholesterol Metabolism: LDLR, SREBF1.
Fatty Acid Metabolism: ACACA, ACOX1, FASN.
Lipid Transport: LDLR, SORBS1.
Other Lipid Metabolism Genes: ARAF, BRAF, INS, LEP, PPARG, PRKCG, PRKCI, PRKCZ, RAF1, SHC1.
Protein Metabolism: Protein Amino Acid Dephosphorylation: DUSP14, PPP1CA, PTPN1, PTPRF.
Protein Amino Acid Phosphorylation: AKT1, AKT2, AKT3, ARAF, BRAF, GSK3A, GSK3B, IGF1R, INSL3, INSR, MAP2K1, MAPK1, PDPK1, PRKCG, PRKCI, PRKCZ, RAF1, RPS6KA1.
Protein Biosynthesis: ANG, EIF2B1, EIF4EBP1, LDLR, PPP1CA.
Protein Transport: PRKCI, RRAS2.
Other Protein Metabolism Genes: AEBP1, CBL, DOK1, DOK2, FRS2, GAB1, IRS1, NOS2, SORBS1, VEGFA.
Transcription Factors and Regulators: DNA-dependent Regulation of Transcription: CEBPA, CEBPB, FOS, JUN, PPARG, SREBF1.
RNA Polymerase II Transcription Factor Activity: CEBPA, FOS, JUN, SREBF1.
Transcription Factors: AEBP1, CBL, CEBPA, CEBPB, JUN, PPARG, SREBF1.
Cell Growth and Differentiation: Cell Cycle: MTOR, HK2, HRAS, IGF1R, IGF2, KRAS, MAPK1, VEGFA. Cell Prolifeion: GAB1, IGF1R, IGF2, IRS2, NCK1, NPY, PRL, RAF1, SHC1, VEGFA.
Growth Factors and Receptors: GAB1, GRB2, IGF1R, IGF2, IGFBP1, INSR,SHC1, VEGFA.
Cell differentiation: NOS2, PPARG.
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文献和实验PCR芯片，研究者可以同时研究大量基因，既可以进行某些特别感兴趣的信号通路的研究，也可以作为验证芯片结果的方法。 为了获得理想的实验结果，PCR芯片面临以下挑战：高特异性，高灵敏度和可重复性。高灵敏度或低检出限，在样品的总RNA量少，基因表达量低的情况下，也能检测出目的基因。高特异性，扩增产物严格保证基因特异性，避免非特异性产物，如引物二聚体的产生。可重复性，通过标准化的反应体系和实验方法，使得多人多次重复实验均能获得可靠的基因定量结果。 PCR芯片实验操作步骤 采用PCR芯片进行实验只需
Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates
This chapter describes techniques to successfully detect tyrosine phosphorylation of the insulin receptor and insulin receptor substrate proteins. These assays demonstrate whether the insulin signaling pathway is activated at its earliest
【求助】什么是micro-array analysis，怎么翻译？
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