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HIF1 & Co-Transcription Factors: ARNT, COPS5, HIF1A, HIF1AN, HIF3A, HNF4A, NCOA1, PER1. Other HIF1 Interactors: APEX1, EGLN1, EGLN2, NFKB1, P4HA1, P4HB, TP53.
Responsive Genes: Angiogenesis: ADORA2B, ANGPTL4, ANXA2, EGR1, EDN1, EPO, F3, GPI, HMOX1, JMJD6, LOX, MMP9, PGF, PLAU (uPA), SERPINE1 (PAI-1), VEGFA.
Coagulation: ALDOA, ANXA2, F10, F3, F3, PLAU (uPA), SERPINE1 (PAI-1), SLC16A3.
DNA Damage Signaling & RePAir: ATR, MIF, NDRG1, RUVBL2.
Metabolism: ALDOA, DDIT4 (REDD1), ENO1, ERO1L, GBE1, GPI, GYS1, HK2, LDHA, PDK1, PFKFB3, PFKFB4, PFKL, PFKP, PGK1, PKM2, SLC2A1, SLC2A3, TPI1.
Regulation of Apoptosis: ADM, BNIP3, BNIP3L, DDIT4 (REDD1), IER3, MIF, NOS3 (eNOS), PIM1.
Regulation of Cell Prolifeion: ADM, BLM, CCNG2, EGR1, IGFBP3, MET, MIF, MXI1, NAMPT, NOS3 (eNOS), ODC1, PGF, PIM1, TXNIP.
Transcription Factors: BHLHE40, FOS, RBPJ, USF2.
Transporters, Channels & Receptors: SLC2A1, SLC2A3, SLC16A3, TFRC, VDAC1.
Other Responsive Genes: ANKRD37, CA9, CTSA, DNAJC5, EIF4EBP1, LGALS3, MAP3K1.
Pathway Activity Signature Genes: ALDOC, ANGPTL4, ANKRD37, BHLHE40, BNIP3, BNIP3L, CA9, FAM162A, HK2, LDHA, NDRG1, P4HA1, PFKFB4, PGK1, SLC2A3, VEGFA.
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文献和实验PCR芯片，研究者可以同时研究大量基因，既可以进行某些特别感兴趣的信号通路的研究，也可以作为验证芯片结果的方法。 为了获得理想的实验结果，PCR芯片面临以下挑战：高特异性，高灵敏度和可重复性。高灵敏度或低检出限，在样品的总RNA量少，基因表达量低的情况下，也能检测出目的基因。高特异性，扩增产物严格保证基因特异性，避免非特异性产物，如引物二聚体的产生。可重复性，通过标准化的反应体系和实验方法，使得多人多次重复实验均能获得可靠的基因定量结果。 PCR芯片实验操作步骤 采用PCR芯片进行实验只需
1 activity in the hypoxic response. Here, we provide methodologies to monitor signaling components both downstream and upstream of the hypoxia-induced mTORC1 inhibitory pathway. These methodologies will serve as valuable tools for researchers seeking
Wnt Signaling in the Early Sea Urchin Embryo
Wnt signaling regulates a remarkably diverse array of cellular and developmental events during animal embryogenesis and homeostasis. The crucial role that Wnt signaling plays in regulating axial patterning in early embryos
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