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PPARα Targets: Adipogenesis: ADIPOQ, LPL, NR1H3, UCP1.
Fatty Acid Metabolism: ACADL, ACADM, ACOX1, ACOX3, ACSL1, ACSL3,
ACSL4, ACSL5, CPT1A, CPT1B, CPT2, CYP27A1, CYP4A11, CYP7A1, EHHADH, FADS2, HMGCS2, PLTP, SCD.
Lipid Transport: ADIPOQ, ANGPTL4, APOA1, APOA5, APOC3, LPL, NR1H3, OLR1.
Insulin Signaling: CPT1A, SORBS1.
PPARβ/δ Targets: Adipogenesis: ADIPOQ, ECH1, FABP4, LPL, UCP1.
Fatty Acid Metabolism: ACAA2, ACADL, ACADM, ACOX1, ACOX3, ACSL1, ACSL3, ACSL4, ACSL5, CPT1A, CPT1B, CPT2, CYP4A11, CYP27A1, CYP7A1, EHHADH, ETFDH, FABP1, FABP3, FABP4, FADS2, GK, HMGCS2, MLYCD, PLTP, SCD, SLC27A1, SLC27A4.
Lipid Transport: ADIPOQ, ANGPTL4, LPL, OLR1.
Cell Prolifeion: HIF1A, ILK, KLF10, PTEN, PYY.
Insulin Signaling: CPT1A, PDPK1, SORBS1.
Others: PDPK1.
PPARγ Targets: Adipogenesis: ADIPOQ, LPL, NR1H3, UCP1.
Fatty Acid Metabolism: ACADL, ACADM, ACOX1, ACOX3, ACSL1, ACSL3, ACSL4, ACSL5, CPT1A, CPT1B, CPT2, CYP27A1, CYP4A11, CYP7A1, EHHADH, FADS2, GK, SCD.
Lipid Transport: ADIPOQ, ANGPTL4, APOE, DGAT1, LPL, NR1H3, OLR1.
Cell Prolifeion: CLU, ELN, HSPD1, TXNIP.
Insulin Signaling: CPT1A, DGAT1, PCK1, SORBS1.
Others: MMP9, PCK1.
PPAR Cofactors: CHD9, CREB1, CREBBP, EP300, FGR, LPIN1, MED1, NCOA3, NCOA6, PPARGC1A (PGC-1a), PPARGC1B (PGC-1ß), PPRC1, PRIC285, SIRT1, SMARCD3, SRC, TGS1.
PPAR Ligand Transport: CD36, FABP1, FABP2, FABP3, FABP4, FABP5, FABP6, FABP7, SLC22A5, SLC27A1, SLC27A2, SLC27A4, SLC27A5, SLC27A6.
PPAR Transcription Factors: PPARA, PPARD, PPARG, RXRA, RXRB, RXRG
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文献和实验简介：什么是PCR芯片？ 实时定量PCR是检测基因表达最灵敏，最可靠的方法。通过在试验过程中，实时监测荧光染料的信号变化，反映样品中的基因拷贝数。实时定量PCR线性范围广（能达到5个数量级），因此可以检测同一样品中表达量极低的基因和表达量很高的基因。但是，由于实时定量PCR需要制备标准曲线和同时分析内参基因，因此，每次实验只能检测少数几个基因的表达水平，若要检测大量基因，则工作量巨大，耗时长久。 通过简单的，经过优化的实时定量PCR体系，如SuperArray的RT2ProfilerTM
Complete Array of HOX Gene Expression by RQ-PCR
, they exhibit a high degree of sequence similarity, particularly in the homeobox domain. HOX gene expression, the HOXOME , can be quantified by real-time quantitative PCR (RQ-PCR) using carefully selected reagents. In practice, an RQ-PCR platform based on Taqman
Genotyping of Synovial Fibroblasts: cDNA Array in Combination with RAP-PCR in Arthritis
arbitrarily primed polymerase chain reaction (RAP-PCR) and cDNA array with defined genes for a highly sensitive analysis of gene expression profiles in RASF using small amounts of total RNA. RNA can be extracted from cultured SF, isolated, and analyzed using
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