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文献和实验E.Z.N.A.® Protocol for Bacteria
flow-through. Then empty the collection tube and centrifuge the spin cartridge for 10 min at 4000-5000 x g to completely dry the HiBind™ matrix. 8. RNA Elution. Transfer column to a clean 15 ml collection tube (Not supplied) and elute RNA with 250
E.Z.N.Z.TM MicroElute RNA Clean-up Protocol
by vortexing. 4. Apply sample from step 3 to HiBind® MicroElute RNA column inserted in a 2 ml collection tube (supplied). The maximum capacity of the spin cartridge is 700 ul. (Larger volumes can be loaded successively.) A precipitate may form
E.Z.N.Z.TM MicroElute RNA Desalting and Concentration protocol
). The maximum capacity of the spin cartridge is 700 ul. (Larger volumes can be loaded successively.) A precipitate may formupon addition of ethanol in Step 3. Vortex and add the entire mixture to the column. With the spin column inside a 2ml collection tube
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