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文献和实验were evaluated using a CE Instruments GC cold on-column injector with a Flame Ionization Detector (FID) and Model AS 800 auto-sampler. All GC parameters were optimized as specified in the method. The CE Instrument automated cold on-column injector and
l with ddH2 O (take the volume of the nuclei into account).2. Incubate at 37℃ for 10 min.3. Lyse the nuclei by adding 1.5 ml of 6M Guanidium Chloride and shear the DNA by passing the sample through a 19 gauge needle on a syringe.4. Extract the lysate
Run-on transcription monitors the regulation of transcription in isolated nuclei. A. Preparation of Nuclei - (do everything at 0℃ to 4℃ in 50 ml tubes) 1. Pellet between 30 and 300 million cells at 1,500 X g for 10 min
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