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文献和实验all but 100 ul of the LB and resuspend pellet. 24. Plate pellet on LB plate with appropriate antibiotic. 25.Incubate inverted at 37 C overnight
1. Weigh 500 mg of glass beads in a 2 ml centrifuge tube, add 0.2-0.5 g soil sample. Add 0.8 ml Buffer SLX Mlus. Vortex at maximum speed for 3-5 minute to lyse samples. For the best result, A Mixer Mill, such as Fastprep-24, Mixer Mill MM 300
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
a 200 μL pipette, transfer the cut pieces to a freshly prepared 24-well MEF culture plate containing human iPSC medium. 6) Incubate the MEF culture plate containing the picked colonies in a 37°C incubator with a humidified atmosphere of 5% CO2
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