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文献和实验Mag-Binds Plant DNA Kit Magnetic 96 Protocol
the grinding jar onto the clamps of the Tissuelyser. Grind tissue at 30Hz for 1-2 minutes. 1. Collect ground plant tissue (start with 50mg for fresh sample and 10mg for dried samples) in a 1.2 ml 96 well plate. Immediately add 600ul Buffer SLX
kb) 1) Add 0.5 x volume of Buffer P3 and 1 x volume of absolute ethanol (room temperature, 96-100% ) to the DNA solution and vortex to mix well. For example, 100 ul of DNA Solution, add 50 ul of Buffer P3 and 100 ul of absolute ethanol
96-well Plate Dual Luciferase Assay--96孔板荧光素酶检测
and resuspend the cells using the 1ml pipette. Trans fer about 50 ul to 200 ul out of 300ul to the 96 well plate depending on the confluency of each well. ( just to make sure the cell numbers of different wells are within 4 fold range) Spin down the cells
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