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文献和实验DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
method (6) and the other gel slice was transferred into microcentrifuge tube and then completely crushed by a disposable pipette tip. In the next step, 100-200 µl volume of phenol/chloroform/isoamyl alcohol (25:54:1) was added to the microcentrifuge tube
Amplification Place 50-100 ng of template DNA in a 0.2-ml PCR tube. Add forward and reverse primers to a final concentration of 0.4 µM each. Add 2.5 µl of 10X PCR amplification buffer (supplied with Taq DNA polymerase). Add dNTPs to a final concentration
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of 100 units/µL). Incubate with gentle shaking overnight at 37°C. 17. Add 40 µL of 20% SDS (to a final concentration of 1.6%). Incubate with shaking for 30 min at 65°C. 18. Transfer the sample to a 15-mL tube containing
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