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文献和实验Normal cells in culture divide a defined number of times before reaching replicative senescence (1 ). The number of divisions that cells can undergo varies with the species of animal and tissue from which the cells are derived. Cells
SV40 is a small simian virus that has been extensively used as a viral vector in mammalian cells (1 –4 ). The viral genome is a double-stranded supercoiled DNA molecule of 5243 bp (Fig. 1A ) that is packaged into a capsid containing
Cell-Free Transcription of SV40
has been investigating the molecular mechanisms by which the genes of SV40 are transcriptionally regulated. To elucidate the biochemical factors involved in the transcriptional regulation of SV40, the development of a cell-free transcription system was required
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