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文献和实验Isolation of Natural Products by Preparative Gas Chromatography
-on approach to preparative gas chromatography (Prep-GC) technique and its use for the isolation of natural products, particularly terpenes and other volatiles. Starting from the classification of GC separations, columns, and stationary phases, sampling
Nitrocellulose-Based Immunoaffinity Chromatography
-bromide activated Sepharose. In fact, for large-scale preparative purifications this is an appropriate method to generate columns with adequate binding capacities and flow properties. However, for many purposes particu-late nitrocellulose (NC
Purification of Antiserum or Ascites by Protein A/G Chromatography
(4) Neutralization Buffer (NB). See recipe below(5) Elution Buffers (pH 2.7 and pH 1.9). See recipes below(6) Centrifuge tubes and microcentrifuge tubes(7) Preparative centrifuge and microcentrifuge(8) pH strips(9) Microtiter plate reader with 600 nm filter(10) Glass
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