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文献和实验Preparation of Yeast DNA Embedded in Agarose Plugs
-melt InCert (or SeaPlaque) agarose in 50 mM EDTA, also prewarmed to 45°C . (This procedure will make 0.5% plugs, which are fairly fragile, but are better if further manipulations like in gelo digest are required. If not, use 2% agarose to make 1% plugs
Preparation of Yeast DNA Embedded in Agarose Plugs
Preparation of Yeast DNA Embedded in Agarose Plugs Anja van Brabant (adapted from Iadonato, S. P., and A. Gnirke. 1996. RARE-cleavage analysis of YACs. Methods Mol Biol 54: 75-85. ) 1. Inoculate a 5
Determination of Household Insecticides in Indoor Air by Gas Chromatography-Mass Spectrometry
consisting of a glass fiber filter (GFF) and two polyurethane foam (PUF) plugs, followed by a high-volume air pump. This combination is used to sample particle-bound compounds (on the GFF) as well as gaseous compounds (on the PUF plugs). GFFs and PUF plugs
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