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文献和实验Aligning Short Sequencing Reads with Bowtie
. 32:11.7.1?11.7.14. © 2010 by John Wiley & Sons, Inc. Keywords: short reads; read alignment; alignment; read mapping; mapping; genome indexing; comparative genomics; software package
Degenerate PCR, a short guide.
and one of your primers span a exon intron junction. How degenerate can PCR primers be and still function? 1000 - 10.000 fold degeneracy is not uncommon. The degeneracy of the primers can be kept "down" by substituting four base wobbles
Obtaining Embryos: hCG Injection
over the head with the butt of your hand and reach over with your index finger and thumb and pinch the legs of the frog together. This will extend the legs of the frog and completely immobilize it. Using your dominant
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