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文献和实验Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)
, results in a much-reduced sensitivity (~106 cfu/ml). This may be improved by co-culturing the test cell line in the presence of an indicator cell line such as Vero (Prod.No. 84113001-1v1). This enrichment step results in a sensitivity of 104 cfu/ml
at 70-80% confluency or as advised on ECACC's cell culture data sheet.Do not allow media to go out of date. Shelf life is only 6 weeks at +4oC once glutamine and serum is added.Avoid water baths from becoming dirty by using Sigma Clean (Prod. No. S5525
Subculture of Suspension Cell Lines
, fungi and mycoplasma and cross contamination with other cell lines. Materials Chloros / Presept solution (2.5g/l) 1% formaldehyde based disinfectant e.g.Virkon,Tegador 70% ethanol in water (Prod. No. R8382 ) Equipment Personal
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