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文献和实验Epitope Mapping by Printed Peptide Libraries
exceeding 50.000 oligonucleotides per cm2. This is mainly due to the monomer-by-monomer repeated consecutive coupling of 20 different amino acids associated with the lithographic methods, which adds up to an excessive number of coupling cycles
Today, lithographic methods enable combinatorial synthesis of >50,000 oligonucleotides per cm2 , an advance that has revolutionized the whole field of genomics. A similar development is expected for the field of proteomics, provided
Genomic (Plant) DNA: Restriction Digestion for Sou
need to weigh the membrane down so it does not roll back upon itself, just don’t contaminate the unused portions. Measure out the size of membrane needed for the transfer. This should be a bit larger than the gel to be blotted; e.g., if the gel is 14cm long
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