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文献和实验Purification of Endotoxin Free mAbs
all steps from here are with room temperature buffers etc. Measure OD280 and adjust to between 1-20 mg/ml in buffer A. You will need to use the minimum amount of Fast Flow S-Sepharose to maximise yield, so estimate the total amount of Ig
sepharose 4B (Sigma) C-9142 5g. This amount swells to 20 ml volume . Swell resin in 10 mM HCl (86 ml of conc. HCl into 100 ml H2 O) for 15 min at room temperature. Have a scintered glass filter ready to wash resin. Pour the swelled resin into the filter
In Situ Hybridization Protocols
coated with TESTA, dried in air for two hours and then stored at -20deg.C. It is sometimes necessary to wash the slides in DEPC-PBS and three changes of DEPC-water before storing at -20deg.C. This depends on both the probe and embedding material used
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