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文献和实验Preparation and Staining of Paraffin Sections
and expertise and is usually performed by a histology or pathology laboratory. While hand processing can be performed according to the following protocol the results may show marked variation in histology quality and antigenicity. Station Time Solution
v468 Chapter 7 通过免疫组化来测定β-连环蛋白核定位
recognizingβ-catenin. 2. Materials 2.1. Tissue Block Preparation 1. Formalin: 4% (w/v) formaldehyde in phosphate-bufferedsaline (PBS). 2. Tissue-dehydration solutions series: 70%, 96%, and 100%(v/v) ethanol. 3. Xylene. 4. Liquid
Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
) Wash Buffer B (from I‐SAGE Long Kit; Invitrogen, cat. no. T5000‐03) 1× First Strand Buffer (Invitrogen)
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