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文献和实验as well), unless the 5' ends are capped; in that case, the RNA must first be de-capped with tobacco acid pyrophosphatase (TAP).and 5' labeling Digoxigenin-UTP RNA Labeling (Frank Bottone Jr.) Preparing 5' end-labeled alkaline hydrolyzed RNA (Jim
Use of the Hemacytometer for the Determination of Cell Numbers
. This will not be a valid assumption unless the suspension consists of individual well-separated cells. Cell distribution in the hemacytometer chamber depends on the particle number, not particle mass. Thus, cell clumps will distribute in the same way as single cells
could be measured by in vitro kinase assay, we suggestWestern blotting as a safer, faster, and easier way to measurethe effect of an inhibitor on GSK-3 kinase activity. This can beachieved through the analysis of the phosphorylation state ofGSK-3 substrates, e.g
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