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文献和实验Use of the Hemacytometer for the Determination of Cell Numbers
. This will not be a valid assumption unless the suspension consists of individual well-separated cells. Cell distribution in the hemacytometer chamber depends on the particle number, not particle mass. Thus, cell clumps will distribute in the same way as single cells
could be measured by in vitro kinase assay, we suggestWestern blotting as a safer, faster, and easier way to measurethe effect of an inhibitor on GSK-3 kinase activity. This can beachieved through the analysis of the phosphorylation state ofGSK-3 substrates, e.g
Use of the Hemacytometer for the Determination of Cell Numbers
. Cell distribution in the hemacytometer chamber depends on the particle number, not particle mass. Thus, cell clumps will distribute in the same way as single cells and can distort the result. Unless 90% or more of the cells are free
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