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文献和实验Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
for completion on a minigel (see Protocol on Analyzing DNA on Acrylamide gels). 4. For 50 μg of DNA, dilute 0.5 μl of the 20 Units/μl concentrated CIP stock in 50 μl of cold ddH2 O. 5. Incubation time is critical; incubate
, and 20 mMTris-HCl, pH 7.5, sterile filtered. 5. Syringe (30–50 mL) with 0.2-μm pore size filter. 2.3.2. Step 2: IMAC 1. Sample: Pooled Wnt3A containing fractions from Section2.3.1 . 2. HiTrap? Chelating, 1-mL column (GE Healthcare, Piscataway
Protocols for ET recombination
temperature is important, usually 60℃ - 62℃ is optimal. Purify the PCR products by Qiagen column and elute with 2x 50 μl dH2O. Add 10x Dpn 1 buffer and 2 μl Dpn 1 (NEB) and digest (to eliminate template DNA) for 1 hour. Extract with Phenol:CHCl
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