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文献和实验Characterization of Whole Body Cholesterol Fluxes in the Mouse
Cotton swabs 6–0 silk suture 20‐G needle
Labelling Drosophila pupae with BrdU
. 6. Repeat steps 3 to 5 until you collect all the prepupae in the bottles. Keep the bottles at certain temperature (typically 25 C) and let the pupae continue developing. 7. Drink some coffee or tee and do the job again after one hour.
microscope; the relevant objectives are the plan neofluor 5X and 40X. Mounted on the scope is a Narishige Model MO-202 micromanipulator, on which is mounted a needle holder hooked up to a Narishige IM300 injector. A nitrogen gas tank is hooked
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