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文献和实验Standard hybridization technique
sulphate (Sigma) Standard hybridisation solution: 6 x SSC,500μg/ml heparin,1% SDS. N.B: A modification used for the hybridisation buffer which can also give extremely good results is a variation on that described by Church and Gilbert
Standard hybridization technique
). You will need: 6 x SSC (0.9M NaCl,90mM sodium citrate,pH 7) 10% SDS 50mg/ml heparin sulphate (Sigma) Standard hybridisation solution: 6 x SSC,500μg/ml heparin,1% SDS. N.B: A modification used for the hybridisation buffer which can also give extremely good results
Stewart Laboratory Standard Operating Procedure
x g, 1700 rpm, 5 min in 15 ml tubes - no faster!), and resuspend in 10 mL 70% cold EtOH. Store at -20°C overnight or for several days Day 2 Pellet cells and wash once with wash buffer (PBS + 0.5% IFS). Pellet and resuspend in 0.5 mL 2M HCl
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