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文献和实验, and the clear side of the case. Put the case in the holder, black side of the case facing black side of the holder. Run the transfer at 100V for 1 hour.3. Meanwhile, prepare 500 ml of AC Buffer (+ Tween):50 ml glycerol (= 10% glycerol)10 ml 5M NaCl (= 100mM NaCl
Proteasome Preparation from Human Brain
Proteasome Preparation from Human Brain You will need:- Chicken gamma globulin (1mg/ml in H2 O). Chicken gamma globulin standards :- Gamma Globulin Standards Final Quantity of Gamma Globulin
Methods for the Detection of D-Amino-Acid Oxidase
were homogenized in 7 mM pyrophosphate buffer (pH 8.3). The homogenates were centrifuged at 550 x g for 5 minutes. The supernatant solutions were used for the assay. The reaction mixture consisted of 0.3 ml of 0.133 M pyrophosphate buffer (pH 8.3) containing
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