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文献和实验3% TREVIGEL PROTOCOL for 3 bp resolution
can be obtained by running the gel in 1X TAE at 5 volts/cm for 2 hours with a 40 cm gel. For SSR marker screening in 20 x 40 cm gels: For 400 ml of gel solution: At room temperature, stir 350 ml 1X TAE buffer in a 1 Liter screw cap bottle
DO stocks from individual amino acids. Mixing growth medium Add 20ml of 40% glucose and 40ml of 10x CSM solution to 200 ml of 2x SD Heat up mixture to 65 degrees in water bath Preparation of LB Agar Melt
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
EGTA stock, pH 8.0) in 100 ml PBS, pH 7.2-7.4 Heat about 80ml H2 O to 600 C and add paraformaldehyde; add 1-2 drops of 10M NaOH to get paraformaldehyde in solution. Cool to room temperature, add 10 ml 10X PBS, adjust pH with HCl, add MgCl
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