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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
40 1 Rail carrier for X26, square 40mm length Newport CN26-40 4
can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix
. Which adhesive? ** Apathy's mounting medium and variants ** Silanized (APES or TES or positively charged) slides ** Polishing undecalcified bone sections. ** Polylysine-coated slides ** Wrinkles in plastic sections ** Wrinkles in paraffin sections
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