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上海希言科学仪器有限公司
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文献和实验. 10% APS25μl25μl TEMED5μl5μl 5ml5ml Preparations of sample: Protein samples are in 1X sample loading buffer (also called Laemmli dye)...ie to 6 μl protein sample, add 3 μl3X Laemmli dye stock. Boil 3 minutes before loading gel.Sample loading buffer
CCMB buffer to make chemical competent cell
on ice 20 minutes Centrifuge again at 4C and resuspend in 20 ml of ice cold CCMB80 buffer. Incubate on ice for 20 minutes Aliquot to chilled screw top 2 ml vials or 50 μl into chilled microtiter plates Store at -80C indefinitely. Flash freezing
人脂蛋白相关磷脂酶A2(Lp-PL-A2)酶联免疫分析(ELISA)
板 1 ×48 1 ×96 2-8℃保存 标准品: 27 μ g/L 0.5ml× 1 瓶 0.5ml× 1 瓶 2-8℃保存 标准品稀释
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