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文献和实验Marcantonio Lab Protocol Manual
cold room. 3) Aliquot culture into 8 pre-chilled sterile 50 ml polypropylene tubes. Let stand on ice for 10 minutes. 4) Centrifuge cells for 7 minutes at 3000 rpm at 4 degrees. 5) Pour off the supernatant and gently resuspend each pellet in 10 ml
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
of humans, and thus is considered relatively safe for gene transfer experiments. The first packaging line commonly in use was the y2 line [5]. It encodes the ecotropic env gene and makes high titers of vectors. However, it can also lead to the production
HIGH RESOLUTION GENETIC FOOTPRINTING
, antipain, aprotinin 0.45 um syringe filters (about 15) 1 ml and 10 ml syringes glutathione-agarose resin (attached through the sulfur, Sigma G4510) G-15 Sephadex Columns (I.D. 15 mm) Connectors, tubing, gradient maker, stir-plate, peristaltic pump
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