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文献和实验, a method is described that allows construction of multiantennary oligonucleotide glycoconjugates on a solid support. A bis(hydroxymethyl)malondiamide?based phosphoramidite that contains two phthaloyl?protected aminooxy groups compatible with normal chain
Genomic (Plant) DNA: Restriction Digestion for Sou
need to weigh the membrane down so it does not roll back upon itself, just don’t contaminate the unused portions. Measure out the size of membrane needed for the transfer. This should be a bit larger than the gel to be blotted; e.g., if the gel is 14cm long
Analys of Genomic DNA by Southern Hybridization
and mark the position of the wells with very-soft-lead pencil. Soak the gel in 5 x SSC for 5 minutes to remove bits of gel or particles from membrane. 5.Place wet membrane on a paper towel of equal size. Immobilize the DNA by UV-crosslinking (100 mJ/cm
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