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文献和实验purch asing a sequencing kit, we use either the T7 Sequencing Kit (Pharmacia, 100 reactions) or the Sequenase 2.0 Sequencing Kit (USB via Amersham in the U.K., 100 reactions). Reactions are performed in sterile 1.5ml microcentrifuge tubes. Primers
Marcantonio Lab Protocol Manual
during the actual procedure. Dilute Labelling Mix: need 2 ul per sample 2 ul Labelling Mix (USB) 8 ul DD-H20 Dilute Sequenase: need 2 ul per sample 7 ul Enzyme Dilution Buffer (USB) 1 ul Sequenase 2.0 (USB) Master Mix
Fluorescent In Situ Transcription in Cells and Tissues
sequence (Monsma et al., ). (A ) Transcript staining is evident as bright fluorescent neurons (arrows) of medium diameter in this 10‐µm‐thick frozen/fixed tissue section of the wild‐type (WT) mouse striatum. (B ) Fluorescence is diminished in the mutant
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