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文献和实验was used to show that gld-1 mRNA is part of the GLD-3/GLS-1/GLD-4 complex in vivo (Schmid et al. 2009 ). The protein co-IP procedure upon which this protocol is based is described in Analysis of In Vivo Protein Complexes by Coimmunoprecipitation
below is one example of differential gene expression analysis of cells obtained from microdissected tissue. 1. Materials Stratagene RAP-PCR kit 5X RT buffer (GenHunter) dNTP, 250 µM (GenHunter) dNTP, 25 µM (GenHunter) RNase inhibitor (Perkin
Streptomyces:Protocols/Spore Prep
such as: new stocks; phenotypic analysis; conjugations; spore titre, etc. Prerequisites You should have an idea whether or not you strain sporulates. If it does not, then you may only be able to create a cell suspension glycerol of colony forming units
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