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文献和实验Degenerate PCR, a short guide.
tool to find "new" genes or gene families. Most genes comes in families which share structural similarities. By aligning the protein sequences from a number of related proteins you can find which parts of the protein is conserved or which is variable
Embryo Dissection and Micromanipulation Tools
or hand-pulled capillaries. The needles are mounted in holders used for tungsten needles (available from Fine Science Tools or Carolina Biological). The fine-needle tips should be broken off to provide a tool sturdy enough for dissection
upstream to the 5′ end of the PAM( red triangles ). Parts of this fi gure are adapted from [ 19 , 41 ] The guide sequence within sgRNA has a length of 20 bp andis the exact complementary sequence of the target site within thegenome
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