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文献和实验Use of Polarized PC12 Cells to Monitor Protein Localization in the Early Biosynthetic Pathway
methods are required to facilitate their localization. Differentiating rat pheochromocytoma (PC12) cells reorganize their biosynthetic membrane compartments as they develop neurite-like processes. The authors recently showed that polarization
Detection of MicroRNAs and Assays to Monitor MicroRNA Activities In Vivo and In Vitro
). siRNAs are processed from double-stranded RNA (dsRNA), bind to Argonaute proteins, and could assemble with additional proteins to form complexes termed RNA-induced silencing complexes (RISCs) (12 ). Ago-2 is the endonuclease that cleaves the RNAs
Determining the Activation of Rho as an Index of Receptor Coupling to G12/13 Proteins
growth. Therefore, Rho activity can often represent a sensitive marker of G12/13 activity. Here, we describe the Rho activation assay to monitor the activity of G12/13 proteins.
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