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- 文献和实验
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上海希言科学仪器有限公司
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文献和实验mass spectrometer with turbo-ion spray source (Applied Biosystems, Foster City, CA); 10-port diverter/switching valve (Valco, Houston, TX); HPLC system (Agilent Technologies series 1100, Wilmington, DE); 10 mm (C18 ) guard cartridge (Perkin Elmer
E-Z 96 X-press Plasmid Spin Protocol
-well plate. Transfer the cell lysate to each well of E-Z 96 X-press plate. 3. Centrifuge at 3000 x g for 5 minutes at room temperature. Discard the flow-through and re-use the deep well plate. 4. Add 500μl DLW Buffer (diluted with isopropanol
E. Z. N.A.TM X-press Plasmid Protocol
-press spin column inserted in a 2 ml collection tube. 8. Centrifuge at maximum speed (13,000 x g) for 1 minute. 9. Discard the flow-through liquid and re-use the collection tube. 10. Add 500μl DLW Buffer (diluted with isopropanol
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