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文献和实验, and lyophilized as described above, the resulting white powder is dissolved in DMSO to make a 2 mM stock solution. 1) Prepare 500 μL of 20 μM peptoid solution in sheet formation buffer (10 mM Tris-HCl, 100 mM NaCl, pH 8.0 in water) in a 1 dram
be packed down such that it occupies about 0.8ml.Add to the columns 100 μl AC Buffer, spin at 2000 rpm for 3 min. Repeat two more times.7. When the probe labeling reaction is finished, dilute the sample using AC Buffer to make a final volume of 100 μl. Load
SSR GEL and Silver Staining Protocol
μl 10% ammonium persulfate (0.1 g + 1 ml d.i. water) to the acrylamide mix in a beaker, gently mixing well. Then add 50 μl TEMED and mix. Polymerization will not start until TEMED has been added. Do not mix the catalysts together before adding
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