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文献和实验Primer Premier: Program for Design of Degenerate Primers from a Protein Sequence
and often fails due to the presence of secondary structures. Primer Premier automatically scans the target DNA sequences and designs primers in regions of low degeneracy that are free of secondary structures, including hairpins, dimers and false priming sites
Freeze-Drying of Yeast Cultures
with a lyoprotectant. Primary drying is carried out using a centrifuge head connected to a standard freeze-dryer. Once the centrifuge head is running, air is removed and evaporated liquid is captured in the freeze-dryer. Centrifugation continues for 15 min and primary
Streptomyces Phospholipase D Cloning and Production
phospholipids, which do not occur in nature. Specifically, actinomycete PLD recognizes not only the alcohols (i.e., glycerol or serine) corresponding to the polar head groups of natural PLs, but also secondary alcohols, aromatic alcohols, saccharides, N
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