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文献和实验Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
of plasmids containing the viral EBV origin of replication. This protocol describes the instructions for the growth, maintenance and transfection of suspension adapted 293-EBNA cell line using the PEI expression system. Cells can be purchased from ATCC
GENERAL MAINTENANCE INSTRUCTIONS FOR IMAGINAL DISC CELL LINES
GENERAL MAINTENANCE INSTRUCTIONS FOR IMAGINAL DISC CELL LINES This is our lab protocol for growing imaginal disc cells (clone-8) The Milner Lab website has additional protocols for clone-8 cells. Clone 8 cells are passaged
Inverse PCR & Cycle Sequencing
) - CTT GCC GAC GGG ACC ACC TTA TGT TAT T Plw3-1 (19) - TGT CGG CGT CAT CAA CTC C Pwht1 (29) - GTA ACG CTA ATC ACT CCG AAC AGG TCA CA V. Sequencing Generally strong and unique bands result from the above PCR conditions, which can be directly
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