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文献和实验HIGH RESOLUTION GENETIC FOOTPRINTING
column can be loaded many times in order to concentrate the sample. If the concerted integration PCR product has a small amount of smearing, the PCR product should be gel purified using the Qiaquick gel extraction kit as described
Water Maze Escape Learning Task
; Volume =15 cm x (35 cm)2 = 57,727 cc = 57.7 L Surface to rim 5 cm Temperature 25 ± 1 C Opacity For visible platform task - clear Reserve tank Start the day with large volume of fresh water kept at about 27 C with Aquarium heater
Plant DNA methylation analysis by bisulfite genomic sequencing
DNA clean up resin (Promega kit #A7280), vortex (see comment 4); Load resin on provided column, apply vacuum (vacuum flask, membrane pump); Add 2.0ml 80% (v/v) 2-propanol on column, apply vacuum (vacuum flask, membrane pump);
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