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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
production. Following an 8 hour incubation, the filters are marked, removed, washed, blocked and incubated with the AP-fusion protein. Then, the filters are washed again and AP substrate (NBT/BCIP) is added indicating the positive plaques. These positive
Electrophoresing a gel too "hot" can cause the DNA to denature in the gel. It can also cause the agarose gel to deform. Cool the gel with a small fan during the electrophoresis.
Human Placental Alkaline Phosphatase Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike other AP proteins typically expressed by cells. A few cell types (HeLa cells, for example
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