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上海希言科学仪器有限公司
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文献和实验#______________ 1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________ X μl Nuclease free H2O to 10 μl 37℃ at least 1 hr After incubation, bring reaction volume up to 50 μl before cleaning up with spin column
. Hybridization and Exposure to Film 1. Remove tube(s) from hybridization oven. Once the probe has boiled, quick spin it, and add immediately to the prehybridization buffer in the tube. Do not pipette the probe down the side of the tube, as this will cause
Plant DNA methylation analysis by bisulfite genomic sequencing
) at temperature appropriate for used restriction enzyme; Add 0.5ml Tris-buffer-saturated phenol-chroroform, vortex; Centrifuge with Eppendorf 5414 or equivalent at 10000 x g for 2 minutes; Transfer the upper aqueous phase to clean 1.5ml
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