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文献和实验2-D Polyacrylamide Gel Electrophoresis
-Gel casting Chamber (Bio-Rad,#165-2025)as follows: Bottom of chamber Small plate,20 cm Spacers,20 cm x 1 mm Eared plates,20 cm Spacers,20 cm x 1 mm Large plate,20 cm Meylar sheet Repeat as needed. 5.Fill the chamber with acrylic blocks
Healthcare Bio-Sciences Corp. 800 Centennial Avenue P.O. Box 1327 Piscataway, NJ 08855-1327 USA GE Healthcare Bio-Sciences KK Sanken Bldg. 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073 Japan www.gehealthcare.com/protein-purification
Marcantonio Lab Protocol Manual
the four enzyme buffers, as well as the DTT, dNTP mix, PEG, and S1 Stop buffer from the -20 freezer to allow them to thaw. 3) Label 10 Eppendorf tubes 1-10 for the ten time points. Place these tubes on ice. 4) Dissolve the pelleted DNA in 27 ul of DD-H2O. Add
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