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文献和实验He Tube Mating with MSCV recipient
the dilution with the OD closest to 0.1 (this is true for recipient AND donor cultures. You want an actively growing culture for optimal mating) 5. To set up tube mating, you want a 1:1 ratio of recipient:donor. Determine which dilution of the MSCV dilutions
E. Z. N.A.TM X-press Plasmid Protocol
with vigorous shaking until an OD600 of 2.0-4.0 is achieved. 2. Pellet 1-2 mL bacterial culture in a 2.0 ml centrifuge tube by centrifugation at maximum speed (13,000 × g) for 1 min at room temperature. Note: Do not use biomass large
E-Z 96 X-press Plasmid Vacuum Protocol
selective antibiotic. Incubate 14-16 hours at 37℃ 600 with vigorous shaking until an OD of 2.0-4.0 is achieved. 2. Pellet 1-2 mL bacterial culture in a 2.0 ml 96-well deep-well plate by centrifugation at 3000 x g for 15 min at room temperature.
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