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文献和实验Cotton Pistil Drip Transformation Method
could efficiently generate more transgenic plants and allow meaningful analyses of gene function and transgenic plants. Cotton pistil drip by inoculating Agrobacterium tumefaciens onto the pistil after pollination gave rise to stable transformants
and solution is clear. Allow solution to cool to about 55EC before pouring. (Ethidium bromide can be added at this point to a concentration of 0.5 μg/ml) Prepare gel tray by sealing ends with tape or other custom-made dam. Place comb
Southern Blotting: DNA Transfer
#1: 100mM maleic acid, pH 7.5 150mM NaCl Buffer #2: 2% Blocking Reagent in Buffer #1 Heat to 50-70℃; prepare ~60' in advance; sol'n remains turbid Store at 4℃. Add NaAzide for long term storage. Buffer #3: 100mM Tris-Cl, pH 9.5 100mM NaCl 50
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