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文献和实验when temperature of the cover becomes lower than temperature of the liquid in the plate. It is convenient to use an air fan to accelerate thawing. #6. 12.2ml per plate, if PCR volume is 30µl/well (maximum for old ABT-384
Sequencing Reaction Cleanup in 384 Well Sequencing Reaction Plates
1.Take the 384 well viper plate from the thermocycler. Remove the Silicone Sealing Mat (Axygen Scientific: AM-384-PCR-RD) and place face down on a paper towel and pat to absorb any liquid that may be on it. Add 30 μl 95% EtOH/0.12 M NaOAc
when temperature of the cover becomes lower than temperature of the liquid in the plate. It is convenient to use an air fan to accelerate thawing. #6. 12.2ml per plate, if PCR volume is 30µl/well (maximum for old ABT-384
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