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文献和实验A chemokine-driven positive feedback loop organizes lymphoid follicles
-transgenic mice were of the MD4 line 15 . To induce germinal centres, mice were injected intraperitoneally with 100 µg of TNP- or NP-chicken -globulin (Biosearch Technologies) diluted in 200 µl PBS and mixed with the Ribi Adjuvant System (Ribi
Sequencing Reaction Cleanup in 384 Well Sequencing Reaction Plates
1.Take the 384 well viper plate from the thermocycler. Remove the Silicone Sealing Mat (Axygen Scientific: AM-384-PCR-RD) and place face down on a paper towel and pat to absorb any liquid that may be on it. Add 30 μl 95% EtOH/0.12 M NaOAc
by aspiration.4) Add 1.0ml new Lysis buffer. Vortex (15-30s) to break up pellet.6) Centrifuge for 15s at 12,000 rpm. Remove red supernatant by aspiration.** at this stage nuclear pellets may be stored long term by adding 100μl TEN buffer and freezing at 20
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